P Palthera
Signalling Peptides

GHK

Glycyl-histidyl-lysine / Gly-His-Lys

GHK is the uncomplexed form of GHK-Cu — the same (Gly-His-Lys) without bound copper. It is endogenously present in human plasma and declines markedly with age. Most published research discusses GHK in the copper-bound (GHK-Cu) form because the copper binding is central to the proposed mechanisms; this profile distinguishes the uncomplexed peptide for completeness.

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Signalling Peptides
Classification
Glycyl-L-histidyl-L-lysine — endogenous tripeptide (uncomplexed form of GHK-Cu)
Research stage
Mostly in vitro and topical-formulation literature; serum-level decline with age documented
Sequence
Gly-His-Lys
Molecular weight
340.4 Da

Snapshot

Key takeaways

A three-bullet snapshot before reading the full dossier.

  1. 01

    Three- human plasma peptide that declines with age.

  2. 02

    Most published mechanism research uses the copper-bound GHK-Cu form (see separate profile).

  3. 03

    Not approved as a medicine; topical and cosmetic-formulation research is the largest applied use case.

Dossier overview

4

research areas

3

references

3

handling notes

01

Mechanism of action

Free GHK binds Cu(II) ions with high affinity, so much of its biological activity in serum and tissue contexts likely occurs as the GHK-Cu complex. The peptide is associated with collagen synthesis, wound healing, and broad gene-expression effects in cell-culture research.

02

Research applications

  • peptide ageing research
  • Cell-culture and gene-expression research
  • Cosmetic and topical formulation research
  • GHK / GHK-Cu comparison studies

Evidence at a glance

What's behind this profile

3 citations · 2015–2024

Review
3

Narrative or systematic reviews; no primary data.

Publication years

  1. 15
  2. 16
  3. 17
  4. 18
  5. 19
  6. 20
  7. 21
  8. 22
  9. 23
  10. 24
20152024

Counts are derived from the cited studies below. A study covering both in vivo and in vitro work is counted by its primary model. Sample size is reported in 0 of 3 citations. Findings remain model-specific and are not extrapolated to therapeutic use.

03

Study references

Each profile cites a minimum of two peer-reviewed sources, with model type and reported sample size where the source provides it. Findings are model-specific and must not be extrapolated to therapeutic use.

GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration

2015

Pickart L et al. · BioMed Research International

Model
Narrative review of GHK cellular-pathway research
Sample
N/A (review)

Reviewed GHK's reported effects on collagen synthesis, wound healing, and gene-expression patterns documented across the literature.

PMID 26236730 DOI 10.1155/2015/648108

The potential of GHK as an anti-aging peptide

2020

Dou Y et al. · Aging Pathobiology and Therapeutics

Model
Narrative review of GHK biology and ageing research
Sample
N/A (review)

Reviewed the age-related decline of plasma GHK and the antioxidant, anti-inflammatory, and tissue-remodelling properties documented in studies.

PMID 35083444 DOI 10.31491/apt.2020.03.014

Topically applied GHK as an anti-wrinkle peptide: Advantages, problems and prospective

2024

Mortazavi SM et al. · BioImpacts

Model
Narrative review of GHK / GHK-Cu topical anti-wrinkle research
Sample
N/A (review)

Reviewed GHK-Cu and palmitoylated GHK derivatives in anti-wrinkle research, noting that cosmetic-market availability outpaces robust controlled clinical evidence.

PMID 39963574 DOI 10.34172/bi.30071

Evidence caveats

  • Most peer-reviewed research uses the copper-complexed GHK-Cu form. Standalone uncomplexed GHK research is narrower.
  • Cosmetic-market availability does not equate to controlled clinical evidence. GHK is not approved as a medicine.

04

Storage and handling

Store under controlled laboratory conditions, protected from light, moisture, and trace copper contamination.

  • Protect from light and moisture during research storage.
  • Recognise that in solution containing trace copper, GHK will partially complex to form GHK-Cu.
  • Research-only inventory; not for human use.