P Palthera
Synthetic Peptides

PE 22-28

PE22-28 / Shortened spadin analog

PE 22-28 is a shortened analog of spadin (a peptide derived from the propeptide of neurotensin 3 / sortilin) designed by the Mazella / Borsotto group at IPMC-CNRS. It targets the TREK-1 two-pore-domain potassium channel, which has been studied as a depression-associated channel in rodent models.

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Synthetic Peptides
Classification
Shortened spadin analog · TREK-1 channel inhibitor · Preclinical only
Research stage
Preclinical only — rodent and in vitro studies
Sequence
QHFPLPPGHK (positions 22–28 of spadin with capping residues)
Molecular weight
Approximately 1146 Da

Snapshot

Key takeaways

A three-bullet snapshot before reading the full dossier.

  1. 01

    Derived from spadin; reported to inhibit TREK-1 channels in heterologous expression systems.

  2. 02

    All cited evidence is — rodent behavioural models and cell-culture electrophysiology.

  3. 03

    No peer-reviewed human clinical trials are indexed on PubMed.

Dossier overview

3

research areas

2

references

3

handling notes

01

Mechanism of action

PE 22-28 is reported to inhibit TREK-1 (KCNK2) channel currents in heterologous expression systems and produce antidepressant-like effects in rodent behavioural tests, with longer persistence than the parent spadin peptide.

02

Research applications

  • TREK-1 channel pharmacology
  • Rodent forced-swim / tail-suspension behavioural models
  • Cell-culture neurogenesis and synaptogenesis

Evidence at a glance

What's behind this profile

2 citations · 2015–2017

Animal
1

Studies in rodents or other animal models.

Review
1

Narrative or systematic reviews; no primary data.

Publication years

  1. 15
  2. 16
  3. 17
20152017

Counts are derived from the cited studies below. A study covering both in vivo and in vitro work is counted by its primary model. Sample size is reported in 0 of 2 citations. Findings remain model-specific and are not extrapolated to therapeutic use.

03

Study references

Each profile cites a minimum of two peer-reviewed sources, with model type and reported sample size where the source provides it. Findings are model-specific and must not be extrapolated to therapeutic use.

Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity

2017

Djillani A, Pietri M, Moreno S, Heurteaux C, Mazella J, Borsotto M · Frontiers in Pharmacology

Model
In vitro hTREK-1/HEK cells plus in vivo mouse behavioural antidepressant tests
Sample
Not reported in abstract

Reported PE 22-28 inhibited TREK-1 currents in hTREK-1/HEK cells and showed antidepressant-like activity in mouse models with longer persistence than spadin.

PMID 28955242 DOI 10.3389/fphar.2017.00643

Targeting two-pore domain K+ channels TREK-1 and TASK-3 for the treatment of depression: a new therapeutic concept

2015

Borsotto M, Veyssiere J, Moha Ou Maati H, Devader C, Mazella J, Heurteaux C · British Journal of Pharmacology

Model
Narrative review (covers TREK-1 knockout mouse work)
Sample
N/A (review)

Reviews the rationale for TREK-1 / TASK-3 inhibition as an antidepressant strategy, including the spadin lineage that PE 22-28 was developed from.

PMID 25263033 DOI 10.1111/bph.12950

Evidence caveats

  • No controlled human trials indexed on PubMed at time of writing.
  • All published primary evidence originates from the Mazella / Borsotto group at IPMC-CNRS; independent replication is limited.
  • Findings are model-specific (rodent / in vitro) and are not extrapolated to therapeutic use.

04

Storage and handling

Typically stored in a cool, dry environment under controlled laboratory conditions per supplier documentation.

  • Use supplier documentation for batch-specific handling.
  • Limit repeated cycles during research inventory handling.
  • Document date and storage condition where applicable.

Common questions

PE 22-28 FAQ

Plain-English answers backed by the citations on this profile — what it is, what's been studied, regulatory status, evidence limits.

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